How to Create Isometric Tiles with Adobe Illustrator
Mazes 2D Tiles - Tile format and naming conventions
Creating Isometric Tiles with Adobe Illustrator (Video)
This video tutorial will show you the general workflow and some tricks to use Adobe Illustrator's tracing function. This will create SVG files from bitmap files (JPG, PNG). The SVG files can be imported into Puzzle Maker Pro - Connect the Dots.